NCERT SOLUTIONS FOR CLASS 12 BIOLOGY CHAPTER 11 PDF
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These solutions are also available for download in PDF format here. Find NCERT Solutions for Class 12 Biology – Chapter 11, Biotechnology. NCERT Solutions Class 12 Biology Chapter 11 Biotechnology Principles And Processes PDF are provided here for free. These NCERT class 12 biology. Click here to get Class 12 Biology NCERT Solutions Chapter 1 Reproduction In Organisms. The NCERT Solutions for class 12 biology chapter 1 pdf is given below. Class 11Th Maths Ncert Solutions Pdf Download · Ncert Physics Class
Origin of Replication Ori : This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA.
Cloning Sites: A location on a cloning vector into where a foreign gene can be introduced is called recognition site. The vector must have very few preferably single recognition sites. The presence of more than one recognition sites within the vector will produce several fragments which will make the process of gene cloning more complicated. Therefore, the foreign DNA is ligated at a restriction site present in one of the two antibiotic resistance gene.
Selectable Marker: It is a gene which helps in identifying and eliminating non-trans formants from trans formants having recombinant DNA by selectively permitting the growth of trans formants.
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NCERT Solutions For Class 12 Biology Chapter 11
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About Vedantu. Justify your answer. Eukaryotic cells do not have restriction nuclease.
Restriction enzymes restrict the infection of bacteria by some viruses like bacterio phages by degrading the viral DNA without affecting viral DNA. Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks? Stirred tank bioreactors provide a better agitation system, oxygen delivery system, foam system, pH control system, temperature control system and provide a better provision for cleaning and sterilization.
Collect 5 examples of palindromic DNA sequences by consulting your teacher.
Better try to create a palindromic sequence by following basepair rules. Palindromic DNA sequences are a group of letters that form the same word when read from both forward and backward. The linkage of antibiotic resistance gene with the plasmid vector with the plasmid with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends. This makes a new combination of circular autonomously replicating DNA created in vitro and this is known as recombinant DNA.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Class 12 BiologyNCERT Solutions for Biotechnology Principles and Processes
So if a recombinant DNA bearing gene for resistance to an antibiotic is transferred into E. Since due to ampicillin resistance gene,one is able to select a transformed call in the presence of ampicillin.
When we insert a piece of alien DNA into a cloning vector and transfer it into a bacterial, plant or animal cell, the alien DNA gets multiplied. The whole process involves the use of restriction endonucleases, DNA ligase, appropriate plasmid or viral factors to isolate and ferry the foreign DNA into host organisms. When a piece of DNA gets integrated into the genome of the recipient, it may multiply and be inherited along with the host DNA.
NCERT Solutions Class 12 Biology Chapter 11 – Biotechnology: Principles And Processes
This happens because the alien piece of DNA has become part of a chromosome, which has the ability to replicate.
In a chromosome there is a specific DNA sequence called the origin of replication, which is responsible for initiating replication.
Thus, an alien DNA is linked with the origin of replication, so that, this alien piece of DNA can replicate and multiply itself in the host organism. Bioreactors are vessels in which raw materials are biologically converted into specific products, individual enzyme, etc.
A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions temperature, pH, substrate, salts, vitamins, oxygen.
NCERT Solutions for Class 12 Biology Chapter 11
After the biosynthetic stage, the products are subjected through a series of processes, such as separation and purification, before it is ready for marketing as a finished product. PCR means polymerase chain reaction.
In this reaction multiple copies of gene of interest is synthesised in vitro using two sets of primers and the enzyme DNA polymerase. The enzyme extends the primers using the nucleotides provided in the reactuion and the genome DNA as template.University of California, Davis. Justify your answer. This makes a new combination of circular autonomously replicating DNA created in vitro and this is known as recombinant DNA.
Become a Teacher. Do eukaryotic cells have restriction endonucleases?
Justify your answer. We cover all exercises in the chapter given below:- Chapter 11 Exercises - 12 Questions with Solutions. The stirrer facilitates even mixing and oxygen availability throughout the bioreactor. The ability to multiply copies of antibiotic resistance gene in E.
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