PLANT BIOTECHNOLOGY SLATER PDF
Adrian Slater; Nigel W Scott; Mark R Fowler. Oxford ; New York: Oxford University Press, Plant Biotechnology presents a balanced, objective exploration of the technology behind genetic manipulation, and its application to the growth and cultivation of plants. Throughout history, humankind has pursued means to improve the yield of crop plants through selective plant breeding and hybridization. Few areas of science have progressed as rapidly, or have had such an impact on public consciousness and governments, as recent advances.
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Plant Biotechnology - The Genetic Manipulation of Plants () Oxford University Press, Oxford - Adrian Slater, Nigel Scott, Mark Fowler - Download as PDF. PDF | On Jan 1, , Hani Al-Ahmad and others published book review Plant Biotechnology The genetic Manipulation of book review Plant Biotechnology The genetic Manipulation of Plants . By Adrian Slater, Nigel W. Scott, and Mark R. Request PDF on ResearchGate | On Jan 1, , Adrian Slater and others published Plant Biotechnology.
Organogenesis relies on the inherent plasticity of plant tissues, and is regulated by altering the components of the medium. In particular, it is the auxin BOX 2. When cultured on a medium containing both auxin and cytokinin, callus will proliferate. If the auxin to cytokinin ratio is increased, adventitious roots will form from the callus by organogenesis.
Plant biotechnology : the genetic manipulation of plants
It the auxin to cytokinin ratio is decreased adventitious shoots will be formed. If the explants are cultured on medium containing only a cytokinin shoots can be produced directly.
Tobacco plants can also be easily regenerated from tobacco leaf pieces. Leaves are cut into aproximately 1 cm squares with a sterile scalpel avoiding large leaf veins and any damaged areas.
The leaf pieces are then transferred right side up to gelled MS medium supplemented with 1 mg l-1 BAP a cytokinin and 0. Over the next few weeks, callus forms on the explants, particularly around the cut surfaces.
After 3 to 5 weeks shoots emerge directly from the explants or from callus derived from the explants. When these shoots are about 1cm long they can be cut at the base and placed on to solid MS medium without any plant growth regulators.
The shoots will form roots and form plantlets that will grow in this medium and can subsequently be transferred to soil.
An explant can be a variety of tissues, depending on the particular plant species being cultured. The explant can be used to initiate a variety of culture types, depending on the explant used. Regeneration by either organogenesis or somatic embryogenesis results in the production of whole plants.
The following three chapters provide a further interesting insight into strategies for improving stress tolerance, developing crop yields and quality, and prospects for molecular farming.
The book culminates in a final review of future prospects for genetically modified crops, which stresses the legislative framework together with economic, social and ethical dimensions.
Not only does this break up the text, but provides some useful self-standing information. Further reading information and web-links particularly to on-line journals are usefully given at the end of each chapter.
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More than this, and of special interest, is a dedicated website associated with the book, providing downloadable figures of particular value to lecturers, and update sections. Plant biotechnology is a well thought-out teaching aid, which distinguishes itself in part by its wide-ranging coverage.
As might be anticipated, however, for such a subject, the book is not, in parts, an easy read, particularly the introductory immersion provided in Chapter 1, which requires concentrated effort. The different styles and, in parts, intensity of subject coverage are presumably the result of multi-authorship.
None of this detracts, however, from the value of the text, either in content or the coverage.
A number of sections in the book are particularly noteworthy and informative, including descriptions of the successful development of glyphosate herbicide resistance and the use of insecticidal Bt genes from Bacillus thuringiensis.
Interesting coverage is also provided of the very important Arabidopsis Genome Sequencing Initiative and the International Rice Genome Sequencing Project, which clearly represent milestones in biotechnology discovery.Callus cultures can also be used to initiate cell suspensions.
The ploidy of the plants obtained from haploid cultures may not be haploid. There are three methods of plant regeneration via organogenesis.
Split View Views. Further reading Barcelo. None of this detracts, however, from the value of the text, either in content or the coverage.